LumiScope in vivo microscope imaging systemuse diameter 350 μm ultra-fine optical lens and 10,000imaging optical fibers arranged in arrays using various fluorescent proteins and fluorescent calcium indicators as well as light-sensitive proteins (e.g. ChR2 and NpHR） conduct experiments and implement 2 μm level of spatial resolution. the activity of specific nerves can be controlled through optogenetic stimulation.

the system is supported by an advanced model animal platform.

feature:

* makeuse GRIN lenses and fiber optic imaging

* minimally invasive deep brain imaging;

* measurements under conditions of free movement of animals;

* simultaneous recording from multiple brain areas;

* compatible with various fluorescent proteins and fluorescent probes;

* label-free neural activity imaging through intrinsic fluorescence measurements;

* capillary blood flow measurement;

* fluorescence observation of cells in deep organs in the body;

* high-speed dual-wavelength fluorescence ratio imaging;

* pass △F/Falgorithm background processing in real time/improve signal-to-noise ratio offline;

* use versatile imaging software for control and data analysis;

* optogenetics illuminates multiple target brain areas simultaneously.

LumiScope the optical system part is an ultra-fine fluorescent endoscope ( U-FEIS）， it is dr. mitsuo ouchi koyamauchi from the graduate school of medicine, tohoku university, japan ( Minoru Oyamauchi） exist JST and AMED with the assistance of Lucille Co.，Ltd the result of development carried out by the company.

ultra-fine fluorescent endoscope:

* LED，optogenetics input portx1，laser input portx1

* camera standCseat

* two fluorescence filter cubes can be mounted

* used forSCorRFPfilter setGFPfilter set

* GFP / RFPdual bandpass filter kit for dc

* imaging fiber set2set

* field of view diameter approx.Φ320μmL：1000mmabout10,000

* array imaging fiber* 1

* outer diameterφ450-500μmfixed on metal protective tube* 1

optogenetics:

* light source usageLEDor laser

* irradiation time can be changed for each target

* illuminate multiple targets simultaneously (up to90positions)

* shine light of any wavelength on every target

simultaneous imaging of multiple brain regions

multifield imaging fibers can be inserted into different sections to be measured, allowing the use of one LumiScope photometric measurements were taken simultaneously.up to 9 multipoint imaging fibers can be used.

imaging acquisition software

* easy to set up and operate the camera and LED light source control

* multiple shooting modes: snapshot, time-lapse shooting, streaming media, multi-color imaging

* available online and offline. pass ΔF/ F improved signal-to-noise ratio of images obtained by processing

* luminance analysis and ratio data processing are possible

endoscopic fiber optic imaging microscope

* enables cellular calcium imaging and optogenetics in multiple brain regions

* multi-wavelength fluorescence imaging

* can record on freely moving animals

computer

* OS Windows10 64bit

* desktop or laptop package

* 4ch TTL output

   imaging fibers and lenses are consumables and are not covered by the warranty

   for portable computers, additional optional shutter controller is required

application cases

capture changes in neural activity associated with visual stimuli without labeling

response when an endoscope is inserted into the cortical visual cortex and light stimulation is given to the other eye (fluorometry of flavoproteins)